ABSTRACT
UPLC-Q-TOF-MS and serum pharmacochemistry were employed to study the migrating components in rat sera after intragastric administration of the water extracts of Puerariae Lobatae Radix(PLR) and Puerariae Thomsonii Radix(PTR). After the respective intragastric administration of PLR and PTR extracts, blood samples were collected from the orbital vein. The serum samples were treated by protein precipitation method with methanol and acetonitrile at a ratio of 1∶1 and then passed through Agilent ZORBAX RRHD SB-C_(18) column(3 mm×100 mm, 1.8 μm) and Agilent SB-C_(18) pre-column(3 mm×5 mm, 1.8 μm) with 0.1% formic acid aqueous solution(A)-acetonitrile(B) as the mobile phase. The elution was performed at the flow rate of 0.25 mL·min~(-1), the column temperature of 40 ℃, and the injection volume of 2 μL. By comparison of the total ion chromatogram and secondary fragment ion information of PLR and PTR water extracts, PLR-and PTR-containing sera, and blank serum, we found 42 migrating components(including 17 prototype components and 25 metabolites) in the sera of rats treated with PLR and 35 migrating components(including 15 prototype components and 20 metabolites) in the sera of rats treated with PTR. Thirty-three common components were shared by the two treatments, including 13 prototype components and 20 metabolites. The differences of migrating components in the PLR-and PTR-treated rat sera provide a scientific basis for further study of the active components and quality markers of PLR and PTR.
Subject(s)
Animals , Rats , Drugs, Chinese Herbal , Plant Roots , Pueraria , SerumABSTRACT
Objective:To study the serum pharmacochemistry of Aurantii Fructus （AF）， and to investigate the pharmacological material basis of AF extract in rats. Method:Rapid identification and speculation of the prototype constituents and their metabolites in vivo were carried out according to the relative retention time， accurate relative molecular mass， cleavage fragments of MS/MS and neutral loss of metabolites with ultrahigh performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry （UPLC-Q-TOF/MS） technique by comparing the differences between different samples such as AF extracts， blank plasma， and administered plasma under the same chromatographic and mass spectrometric conditions. Result:After oral administration of the AF extract， 74 transitional constituents absorbed into the blood were detected in serum， in which 49 compounds were prototype constituents and the other 25 were metabolites. The prototype constituents could be divided into dihydroflavones， polymethoxyflavonoids， limonins， coumarins and alkaloids. The identified metabolites included glucuronic acid conjugates， sulfuric acid conjugates， hydroxylated products of flavonoid glycosides and polymethoxyflavonoids， as well as the simultaneous glucuronidation and sulfation products. Conclusion:The constituents absorbed into the blood and their metabolites may be the pharmacodynamic components of AF. Among them， alkaloids， polymethoxyflavonoids and coumarins are mainly introduced into the blood in the prototype form， while naringin and neohesperidin （the index components） exert effect mainly through hydrolysis into aglycones. This work will help to further elucidate the material basis of AF.
ABSTRACT
Objective:To analyze and identify the brain and blood absorption components of rats after intragastric administration of Buyang Huanwu Tang(BYHWT). Method:The brain tissue,plasma of normal rats and the cerebral ischemia-reperfusion rats were analyzed by UPLC-Q-TOF-MS/MS.The prototype components in BYHWT were identified according to retention time,accurate relative molecular weight,primary and secondary mass spectrometry data. Result:After the administration of BYHWT,five compounds were found to enter the normal brain tissue through the blood-brain barrier and identified as calycosin-7-glucoside,albiflorin,formononetin-7-O-β-D-glucoside-6″-O-acetyl,safflower yellow A and astragaloside A;two compounds penetrated the blood-brain barrier and entered modeling brain tissue,and they were identified as calycosin-7-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl;seven compounds entered normal plasma and were identified as calycosin-7-glucoside,albiflorin,hydroxysafflor yellow A,et al;three compounds entered model plasma and identified as calycosin-7-O-β-D-glucoside-6″-O-acetyl,6″-O-acetyl-(6αR,11αR)-9,10-dimetho-xypterocarpan-3-O-β-D-glucoside and formononetin-7-O-β-D-glucoside-6″-O-acetyl. Conclusion:BYHWT has different pharmacological material basis in normal and cerebral ischemia-reperfusion rats.
ABSTRACT
Objective To investigate the prototype components and metabolites of Mentha haplocalyx in rats plasma. Methods UHPLC-Q-TOF-MS/MS method was developed and applied to identify the components and metabolites of rat plasma. The analysis was carried out on an Eclipse Plus C18 column (100 mm × 4.6 mm, 3.5 μm, Agilent) with the mobile phase of 0.1% acetic acid solution (A)-acetonitrile (B) at a flowing rate of 0.3 mL/min, and the injection volume was 5 μL. Results The developed method was applicable to the analysis and identification of metabolites of M. haplocalyx after oral administration. A total of 67 compounds, including 28 prototype components and 39 metabolites (one of which was a new metabolite of luteolin unreported), were identified by comparison of their retention time, ion fragmentation information with that of the blank biological samples, herb extract, and reference compounds. Conclusion The metabolic pathway of M. haplocalyx in rats were consisted of oxidation, reduction, methylation, sulfation, and glucuronidation, and the main metabolic pathway was phase II metabolic pathway among of them. This experimental method is simple and reliable, which could provide theoretical basis for elucidating the bioactive components of M. haplocalyx.
ABSTRACT
In order to evaluate the advantages and problems of serum pharmacochemistry of traditional Chinese medicine (TCM) for screening active ingredients, all literatures concerned from 2003 to 2013 were searched and analyzed. According to our statistics, there were 88 Chinese materia medica (CMM) formulae or single herbs involved; Nine hundred and seventy components were absorbed into blood, including 582 prototype components and 388 metabolites; Among them, 270 prototype components (46.4%) were identified, and only 37 metabolites (9.53%) were identified. Further investigation suggested that 80.37% of the absorbed prototype components identified were pharmacologically active according to relative reports. Thus, the above method is not only fast, accurate, and cost-saving, but also easy to operate, popularize, and utilize in most screening cases of CMM. However, this method has certain limitations at the range of application and problems such as non-standard, non-system, no indepth, low internationalization or industry promotion.